Cranberry study calls for more accurate cranberry authentication testing methods

More accurate cranberry authentication testing methods required?

Cranberry bog photo from above

A fast quality control protocol based on reference BL-DMAC assay and High-performance thin-layer chromatography (HPTLC)-Densitometry was optimized for routine quality control of cranberry (Vacci- nium macrocarpon) products. The sample preparation appeared as a key step to ensure a relevant comparison of products quality and biological data. Sequential solid/liquid extractions were required to fully extract polyphenols, regardless the extraction solvent chosen. The HPTLC-densitometry herein protocol used epicatechin, PAC-A2 and PAC-B2 as quality markers and densitometric measurements at 200 nm. The PAC-A2/Epicatechin ratio, obtained using HPTLC, appeared as an appropriate indicator of the quality of cranberry ingredient. Only two products, among the tested panel, exhibited a high quality cranberry-based ingredient using this criteria. UPLC-MS analyses coupled to multivariate analyses confirmed HPTLC-densitometry conclusions. This work highlighted the plural PAC composition of commercial products and the lack of standardisation for cranberry-based products on the market, explaining the non-reproducibility of clinical trials.

READ FULL STUDY HERE: QUALITY CONTROL OF COMMERCIAL CRANBERRY PRODUCTS

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