Your one-stop shop probiotic solutions provider to target specific health markets

Your one-stop shop probiotic solutions provider to target specific health markets.

Due to their very nature (live microorganisms), probiotic yeast and bacteria are not like any other ingredients and their
formulation requires specific technological and microbiological know-how to ensure their viability, hence optimal efficacy
when delivered to the consumer. This is why probiotic formulation is an art and science, and the selection of the strain(s),
even though a key step, is not sufficient to ensure quality and stability of the finished supplements: manufacturing
processes, from fermentation to freeze-drying and blending, as well as the interaction with other ingredients of the formula
should all be taken into account. Lallemand Health Solutions, which consolidates probiotic expertise and know-how of
Institut Rosell and Harmonium International, totals close to a century of expertise in the selection, development, production
and marketing of probiotic strains and formulations that support human health and well-being. Thanks to its fully integrated
offer, the company can provide turnkey and custom probiotic solutions from fermentation and lyophilisation all the way
to finished product, a true one stop shop probiotic solutions provider. This article reviews the criteria and possibilities to
create successful customized probiotic supplements.
What to look at when choosing a probiotic strain? Single or multiple strain formula?

The first point to consider when developing new probiotic formula
is the choice of the manufacturer and the strain(s) according
to the desired positioning. Attention should be drawn to the
documentation provided by the manufacturer about its strains
properties and ingredients.
A probiotic strain product should be:
• Characterised: e.g. microscopic procedures, metabolic profiling
and advanced genetic techniques to determine strain identity,
those are systematically performed at Lallemand Health Solutions.
• Safe, for the consumer and the environment: absence of
antibiotic resistance gene or transposable genetic material.
• Alive: it should survive processing, storage and digestion. Not
all strains are equal with regard to gastric acidity survival (Fig. 1).
In case of poor acidic resistance of a strain which is interesting in
terms of health benefits, adapted protective technologies can be
applied, as discussed below.
• Effective: specific health benefits and modes of action should be
documented.
Moreover, it is important to select the product from a reputable
provider with high quality standard assurance. The purity (absence
of chemical and microbiological contaminants) is particularly
important.
Figure 1: Resistance to acidity (pH 2) of various Rosell probiotic
strains (% of survival after incubation at pH 2 for 90 minutes
(Lactobacilli) and 60 minutes (Bifidobacteria).
One of the first questions when formulating a probiotic is whether
to go for a single or multiple strain product. Beyond the trends,
there are some pros and cons to consider for both options. The
arguments are numerous for a multi-strain option:
• The intestinal microflora contains 1000 different bacterial species
and numerous strains!
• Different microbes reside in different niches of the intestinal
tract, thus the association of several species can have an effect at
various levels along the gut, while a single strain cannot reside in
all niches (e.g. Lactobacilli and Bifidobacteria)
• Probiotic strains exert multiple modes of action; the association
of various strains can have a synergistic effect.
However, all strains are not always compatible with each other,
and some even exert contradictory effects; some strains are more
stable than others. Moreover, too many strains used in the same
formulation can make it difficult for the bacteria to stabilise.
Thus, it is crucial to find the right balance when formulating
a multiple strain product and to rely on the manufacturers
experience and know-how in order to ensure the optimal health
benefits and stability of the finished product.
The activity and efficacy of probiotics depend on their viability in
the gastrointestinal tract of the host, their primary site of action.
From the manufacturer’s plant to the consumer’s gut, they have to
survive many stresses. Their resistance to these stresses is linked
to various factors, internal (see Fig. 1: not all strains are equal
with regards to resistance against gastric acidity), and external,
including their own production conditions. In order to ensure the
optimal survival and efficacy of the chosen probiotics, appropriate
protective technologies can be applied.
Over the years, Lallemand Health Solutions has developed several
protective technologies to meet manufacturers needs and allow
a large range of applications and innovative galenic forms. These
technologies are today integrated within an advanced three-level

• 1st level: BIO-SUPPORT™ strain technology: the alliance of
optimal strain selection for a natural resistance to gastric acidity
and a proprietary protection matrix applied during the production
of freeze-dried bacteria. This technology offers superior protection
during manufacturing process, shelf life and digestion (Figure 2).
• 2nd level: PROBIOCAP®: a patented probiotic microencapsulation
technology, ideal in case of drastic processing conditions and
versatile formulations.
• 3rd level: STAR®: an award-winning enteric-coating technology
for capsule formulations, ensuring optimal delivery in the gut for all
probiotic strains (Figure 3).
Figure 3: STAR® enteric coating process
These three exclusive technologies can be used separately or
in combination to formulate a wide range of premium probiotics
solutions.
If gut health is the primary target for probiotics, and the most
documented to date, as we start to discover the variety of
mechanisms of action of probiotics and the involvement of the
digestive microflora in various body functions, probiotics array of
applications has also widened. Today, areas such as immunity,
stress, oral care, women’s health etc. also benefit from scientific
back-up for certain specific strains and blends.
Concerning gut health, it is evident that many of the probiotic
typical modes of action can contribute to gastro-intestinal
microflora balance and digestive health and well-being. By
selecting strains that exert different and complementary activities,
it is possible to formulate specific probiotics for targeted digestive
applications such as microflora balance, diarrhea prevention
(antibiotic associated, traveller’s or acute diarrhea…), IBS
symptoms, lactose intolerance etc.
Immunity and the strengthening of immune defences, in particular
for exposed populations (children, senior…) is a growing area of
application for probiotics. This is not so surprising considering
that the gut harbours around 70% of the body immune defences!
Growing scientific evidence shows that, thanks to different
mechanisms, probiotics are able to modulate the immune
response at various levels (effects are known on both the innate
and adaptive arm of the immune response). The beneficial effects
of probiotics on the immune system are strain dependent. For
example, Lactobacillus Rosell-52 is able to induce the production
of antibodies in a non specific fashion thanks to a mitogenic effect
on B cells, thus increasing the pool of antibodies in the gut lumen
(Easo et al., 2002).
Even more recent is the application of probiotics in the mood and
stress area. One day or another, everybody has experienced the
surprising relationship between the brain and the gut, which is also
sometimes called our “second brain”. The bidirectional dialogue
that coordinates brain and gut functions has been described as
the ‘Brain-Gut Axis’. It now appears that the microbiota, and hence
probiotics capable to interact with this microbiota, play a role in this
dialogue. Recent studies have shown that microbiota manipulation
can even influence behaviour in animals (Bercik et al., 2011).
Lallemand released the first clinical studies showing that a probiotic
blend (L. helveticus Rosell-52 and B. longum Rosell-175), can
alleviate both physiological and psychological signs of stress and
anxiety (Diop et al., 2008; Messaoudi et al., 2011).
Other emerging areas of interest for probiotics applications
are linked to the presence of local microfloras and the ability of
probiotics to interact with those: the mouth (oral care), the vagina
(woman’s health), the skin (allergies ; atopic dermatitis)…
When selecting probiotic strain(s) for a target indication, preclinical
and clinical data, when available, should be carefully studied
and compared. Certain strains show complementary modes of
action or effects and their combination can help offer optimal
efficacy for one specific indication or even multiple benefits for a
more generic indication (e.g. ‘digestive health’).
Table 1: Overview of documented health benefits of some of the
main probiotic strains (bacteria & yeast) from Lallemand Health
Solutions collection.
probiotic protection program:
Figure 2: BIO-SUPPORT™ strain protection technology

 

• 1st level: BIO-SUPPORT™ strain technology: the alliance of
optimal strain selection for a natural resistance to gastric acidity
and a proprietary protection matrix applied during the production
of freeze-dried bacteria. This technology offers superior protection
during manufacturing process, shelf life and digestion (Figure 2).
• 2nd level: PROBIOCAP®: a patented probiotic microencapsulation
technology, ideal in case of drastic processing conditions and
versatile formulations.
• 3rd level: STAR®: an award-winning enteric-coating technology
for capsule formulations, ensuring optimal delivery in the gut for all
probiotic strains (Figure 3).
Figure 3: STAR® enteric coating process
These three exclusive technologies can be used separately or
in combination to formulate a wide range of premium probiotics
solutions.
If gut health is the primary target for probiotics, and the most
documented to date, as we start to discover the variety of
mechanisms of action of probiotics and the involvement of the
digestive microflora in various body functions, probiotics array of
applications has also widened. Today, areas such as immunity,
stress, oral care, women’s health etc. also benefit from scientific
back-up for certain specific strains and blends.
Concerning gut health, it is evident that many of the probiotic
typical modes of action can contribute to gastro-intestinal
microflora balance and digestive health and well-being. By
selecting strains that exert different and complementary activities,
it is possible to formulate specific probiotics for targeted digestive
applications such as microflora balance, diarrhea prevention
(antibiotic associated, traveller’s or acute diarrhea…), IBS
symptoms, lactose intolerance etc.
Immunity and the strengthening of immune defences, in particular
for exposed populations (children, senior…) is a growing area of
application for probiotics. This is not so surprising considering
that the gut harbours around 70% of the body immune defences!
Growing scientific evidence shows that, thanks to different
mechanisms, probiotics are able to modulate the immune
response at various levels (effects are known on both the innate
and adaptive arm of the immune response). The beneficial effects
of probiotics on the immune system are strain dependent. For
example, Lactobacillus Rosell-52 is able to induce the production
of antibodies in a non specific fashion thanks to a mitogenic effect
on B cells, thus increasing the pool of antibodies in the gut lumen
(Easo et al., 2002).
Even more recent is the application of probiotics in the mood and
stress area. One day or another, everybody has experienced the
surprising relationship between the brain and the gut, which is also
sometimes called our “second brain”. The bidirectional dialogue
that coordinates brain and gut functions has been described as
the ‘Brain-Gut Axis’. It now appears that the microbiota, and hence
probiotics capable to interact with this microbiota, play a role in this
dialogue. Recent studies have shown that microbiota manipulation
can even influence behaviour in animals (Bercik et al., 2011).
Lallemand released the first clinical studies showing that a probiotic
blend (L. helveticus Rosell-52 and B. longum Rosell-175), can
alleviate both physiological and psychological signs of stress and
anxiety (Diop et al., 2008; Messaoudi et al., 2011).
Other emerging areas of interest for probiotics applications
are linked to the presence of local microfloras and the ability of
probiotics to interact with those: the mouth (oral care), the vagina
(woman’s health), the skin (allergies ; atopic dermatitis)…
When selecting probiotic strain(s) for a target indication, preclinical
and clinical data, when available, should be carefully studied
and compared. Certain strains show complementary modes of
action or effects and their combination can help offer optimal
efficacy for one specific indication or even multiple benefits for a
more generic indication (e.g. ‘digestive health’).
Table 1: Overview of documented health benefits of some of the
main probiotic strains (bacteria & yeast) from Lallemand Health
Solutions collection.